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課程名稱 |
蛋白質養晶概論與實作
Practical Protein Crystallization |
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開課學期 |
112-2 |
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授課對象 |
生命科學院 生化科技研究所 |
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授課教師 |
楊啟伸 |
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課號 |
BST7008 |
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課程識別碼 |
B22EM0150 |
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班次 |
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學分 |
1.0 |
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全/半年 |
半年 |
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必/選修 |
必修 |
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上課時間 |
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上課地點 |
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備註 |
本課程以英語授課。碩士班A群組必修10學分課程之一。請先與授課教師聯絡。
限碩士班以上
總人數上限：20人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
The content of this course will be based on the scientific background of protein crystal culture, crystal culture principles, crystal culture equipments and crystal culture practice.
Students will first receive three lectures on the scientific theory of protein crystal growth, and then practice crystal growth in person and make long-term records and observations.
It is hoped that after completing this course, students will have a full understanding of the actual experimental procedures required for protein crystal growth and the developments of current crystal culture technology.
本課程內容將以蛋白質養晶之科學背景、養晶原理、養晶設備和養晶實作為主要內容。學生將會先接受三次蛋白質養晶科學理論之演講，之後親自實際養晶，並做長期之記錄和觀察。希望在完成本課程之後，學生能對蛋白質養晶所需之實際實驗程序和現今養晶技術之發展有充分的認知。 |
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課程目標 |
This course mainly introduces the basic theory and practice of protein crystal growth.
At a time when structural biology has received potential attention from life sciences, this course hopes to help graduate students understand:
1) the principles and processes of protein crystal growth, and then
2) have the willingness and
3) ability to actually carry out protein crystal growth.
本課程主要是介紹蛋白質養晶之基礎理論和實作。在結構生物學潛在已受到生命科學重視之際，本課程希望能幫助研究生瞭解蛋白質養晶之原理和過程，進而有實際進行蛋白質養晶之意願和能力。 |
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課程要求 |
1. This class will be taught in English.
2. This is a weekly class, and the fixed weekly day for this class is yet to be discussed in the week 1 class.
3. You will need a computer with Microsoft Excel (any version) installed for one of the classes.
4. In the actual crystal growing experience, providing your own protein samples for crystallization screening is highly recommended. |
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預期每週課後學習時數 |
1 |
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Office Hours |
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指定閱讀 |
Manuel Benvenuti and Stefano Mangani (2007). Crystallization of soluble proteins in vapor diffusion for x-ray crystallography. Nature Protocols, Vol.2, No.7, p.1633-1651. |
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參考書目 |
1. Terese Bergfors. Protein Crystallization Techniques, Strategies, and Tips. A Laboratory Manual. International University Line, La Jolla USA. 1999 (ISBN 0-9636817-5-3) |
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評量方式
(僅供參考) |
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針對學生困難提供學生調整方式 |
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上課形式 |
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作業繳交方式 |
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考試形式 |
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其他 |
由師生雙方議定 |
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